US 10,695,446 B2 · Composition and method for detecting hypoxia

Abstract

A compound and method for detecting hypoxic cells and tissue are provided. The compound includes a probe selected from the group consisting of a hypoxia sensitive 2-nitroimidazole containing fluorescence imaging probe, a hypoxia sensitive reversible ON-OFF fluorescence imaging probe, a hypoxia sensitive azo-based fluorescence imaging probe, and combinations thereof. The method includes contacting the cells or tissue with the probe of any one of claims 1-13 and detecting fluorescent intensity of the cell or tissue, wherein increased fluorescent intensity indicates that the cells or tissue is hypoxic. Also provided are a method of synthesizing the compound and a method for synthesizing a therapeutic agent including the compound.

Claims17

Dependent claims

A compound for detecting hypoxic cells and tissue, the compound comprising a probe selected from the group consisting of a hypoxia sensitive 2-nitroimidazole containing fluorescence imaging probe, a hypoxia sensitive reversible ON-OFF fluorescence imaging probe, a hypoxia sensitive azo-based fluorescence imaging probe, and combinations thereof;

(i) wherein the hypoxia sensitive 2-nitroimidazole containing fluorescence imaging probe comprises a structure selected from the group consisting of:
(ii) wherein the hypoxia sensitive reversible ON-OFF fluorescence imaging probe comprise the structure:
and
(iii) wherein the hypoxia sensitive azo-based fluorescence imaging probe comprises a structure selected from the group consisting of:

1 more element

Dependencies are inferred from the claim text.

Facts

Application

15/567,355Filed 2 May 2016 · us provisional application

Priority

1 May 2015

Published

US 2018/0085475 A129 Mar 2018

Granted

30 Jun 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification37 codes

A61K 49/0052
Characterised by the carrier molecule carrying the fluorescent agent: small organic molecules
A61K 31/045
Medicinal preparations containing organic active ingredients: hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
A61K 31/05
Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates: phenols
A61K 31/19
Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids: carboxylic acids, e.g. valproic acid

Composition and method for detecting hypoxia

Life of this patent

Composition and method for detecting hypoxia

Life of this patent

Abstract

Claims17