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US 10,690,635 B2Granted
Purification of glucagon-like peptide 1 analogs
AssigneeBachem Holding AG
InventorsAndreas Stadelmaier, Ralph O. Schoenleber, Daniel Samson, Frank Dettner
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Abstract
The present invention refers to a method of purifying a glucagon-like peptide 1 analogs, the method comprising a two dimensional reversed phase high performance liquid chromatography protocol, wherein the first step is carried out at a pH value between 7.0 to 7.8 using a mobile phase comprising a phosphate buffer and acetonitrile, and the second step is carried out at a pH value below 3.0 using a mobile phase comprising trifluoroacetic acid and acetonitrile.
Claims20
A method for the purification of Liraglutide, comprising:
- a) providing a liquid composition C comprising crude Liraglutide and at least one unwanted component;
- b) subjecting the composition C to a first reversed phase high performance liquid chromatograph (RP-HPLC) purification at a pH between 7.0 and 7.8, wherein a hydrocarbon bonded silica is used as a stationary phase, a mobile phase comprising an aqueous phosphate buffer AB1 and acetonitrile is used, and elution is effected by gradually increasing the acetonitrile concentration within the mobile phase while collecting Liraglutide containing fractions;
- and
- c) subjecting the pooled Liraglutide containing fractions obtained in step b) to a second reversed phase HPLC purification at a pH below 3.0, wherein a hydrocarbon bonded silica is used as a stationary phase, a mobile phase comprising trifluoroacetic acid and acetonitrile is used, and elution is effected by gradually increasing the acetonitrile concentration within the mobile phase while collecting fractions containing purified Liraglutide.
1 more element
Dependencies are inferred from the claim text.