# US 10,690,595 B2 · Optical sensing device, method of manufacturing the same, and optical sensing method

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- Number: US 10,690,595 B2
- Document ID: US10690595B2
- Status: Granted
- Kind: B2
- Application number: 16/319,155
- Filed: 2017-07-18
- Published: 2019-09-19
- Granted: 2020-06-23
- Earliest claimed: 2016-07-20
- Assignees: Agency for Science, Technology and Research
- Applicants: Agency for Science, Technology and Research
- Inventors: Chi Lok Wong; Malini Olivo
- Web page: https://patentgrep.com/patent/US10690595B2

## CPC

- G01N 21/553: {and using surface plasmons (fluorescence excitation G01N21/648; enhanced Raman G01N21/658)}
- G01J 3/0224: {using polarising or depolarising elements}
- G01J 3/447: Polarisation spectrometry
- G01J 4/00: Measuring polarisation of light
- G01N 21/21: Polarisation-affecting properties (G01N21/19 takes precedence)
- G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}
- G01N 21/05: Flow-through cuvettes (G01N21/09 takes precedence; handling fluid samples G01N1/10)
- G01N 2021/212: {Arrangement with total internal reflection}
- G01N 2021/7753: {Reagent layer on photoelectrical transducer}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N)

## Abstract

Various embodiments may provide an optical sensing device based on surface plasmon resonance (SPR). The optical sensing device may include an optical arrangement configured to provide a first polarization light beam and a second polarization light beam, and a first optical member including a sensing surface, the first optical member configured to receive the first and second polarization light beams and reflect the first and second polarization light beams at the sensing surface. The optical sensing device may further include a second optical member arranged to receive the reflected first and second polarization light beams from the first optical member and configured to separate the reflected first and second polarization light beams in a first direction and a second direction, respectively. The optical device may additionally include a detector arrangement configured to detect the reflected first and second polarization light beams from the second optical member.

## Claims

### Claim 1

An optical sensing device based on surface plasmon resonance (SPR), the optical sensing device comprising:
an optical arrangement configured to provide a p-polarization light beam and a s-polarization light beam;
a prism comprising a sensing surface at a base of the prism, the prism configured to receive the p-polarization light beam and the s-polarization light beam and reflect the p-polarization light beam and the s-polarization light beam at the sensing surface;
a polarizing beam splitter arranged to receive the reflected p-polarization light beam and the reflected s-polarization light beam from the prism and configured to separate the reflected p-polarization light beam and the reflected s-polarization light beam in a first direction and a second direction, respectively; and
a detector arrangement configured to detect the reflected p-polarization light beam and the reflected s-polarization light beam from the polarizing beam splitter;
wherein the optical arrangement comprises:
a light source configured for emitting a light beam comprising a p-polarization component and a s-polarization component; and
an initial beam splitter arranged between the light source and the prism, the initial beam splitter configured to receive the light beam from the light source and configured to separate the light beam into the p-polarization light beam and the s-polarization light beam;
wherein the light source is configured to be at a predetermined orientation for emitting the light beam comprising the p-polarization component and the s-polarization component, the predetermined orientation being 1° to less than 45° from an orientation of the light source which produces a light beam without the s-polarization component.

### Claim 2 (depends on 1)

The optical sensing device according to claim 1, wherein the detector arrangement comprises a first detector arranged to detect the reflected p-polarization light beam and a second detector arranged to detect the reflected s-polarization light beam.

### Claim 3 (depends on 1)

The optical sensing device according to claim 1, wherein the p-polarization light beam is modifiable by a surface plasmon wave propagating at the sensing surface during SPR and defines a sensing signal, and wherein the s-polarization light beam is at least substantially unmodifiable by the surface plasmon wave propagating at the sensing surface during SPR and defines a reference signal.

### Claim 4 (depends on 1)

The optical sensing device according to claim 1, further comprising:
a processor communicatively coupled to the detector arrangement, the processor configured to provide a sensing output based on a difference between the reflected p-polarization light beam and the reflected s-polarization light beam.

### Claim 5 (depends on 4)

The optical sensing device according to claim 4, wherein the difference is a difference in an intensity of the p-polarization light beam and an intensity of the s-polarization light beam.

### Claim 6

A method of manufacturing an optical sensing device based on surface plasmon resonance (SPR), the method comprising:
providing an optical arrangement configured to provide a p-polarization light beam and a s-polarization light beam;
providing a prism comprising a sensing surface at a base of the prism, the prism configured to receive the p-polarization light beam and the s-polarization light beam and reflect the p-polarization light beam and the s-polarization light beam at the sensing surface;
providing a polarizing beam splitter arranged to receive the reflected p-polarization light beam and the reflected s-polarization light beam from the prism and configured to separate the reflected p-polarization light beam and the reflected s-polarization light beam in a first direction and a second direction, respectively; and
providing a detector arrangement configured to detect the reflected p-polarization light beam and the reflected s-polarization light beam from the polarizing beam splitter;
wherein the optical arrangement comprises:
a light source configured for emitting a light beam comprising a p-polarization component and a s-polarization component; and
an initial beam splitter arranged between the light source and the prism, the initial beam splitter configured to receive the light beam from the light source and configured to separate the light beam into the p-polarization light beam and the s-polarization light beam;
wherein the light source is configured to be at a predetermined orientation for emitting the light beam comprising the p-polarization component and the s-polarization component, the predetermined orientation being 1° to less than 45° from an orientation of the light source which produces a light beam without the s-polarization component.

### Claim 7 (depends on 6)

The method according to claim 6, wherein the detector arrangement comprises a first detector arranged to detect the reflected p-polarization light beam and a second detector arranged to detect the reflected s-polarization light beam.

### Claim 8 (depends on 6)

The method according to claim 6, wherein the p-polarization light beam is modifiable by a surface plasmon wave propagating at the sensing surface during surface plasmon resonance (SPR) and defines a sensing signal, and wherein the s-polarization light beam is at least substantially unmodifiable by the surface plasmon wave propagating at the sensing surface during surface plasmon resonance (SPR) and defines a reference signal.

### Claim 9 (depends on 6)

The method according to claim 6, further comprising:
a processor communicatively coupled to the detector arrangement, the processor configured to provide a sensing output based on a difference between an intensity of the reflected p-polarization light beam and the reflected s-polarization light beam.

### Claim 10

An optical sensing method based on surface plasmon resonance (SPR), the method comprising:
providing a sample on a prism, the prism comprising a sensing surface on which the sample is provided, the sensing surface at a base of the prism;
providing, by an optical arrangement, a p-polarization light beam and a s-polarization light beam to the prism;
reflecting the p-polarization light beam and s-polarization light beam at the sensing surface to provide a reflected p-polarization light beam and a reflected s-polarization light beam;
separating, by a polarizing beam splitter, the reflected p-polarization light beam and the reflected s-polarization light beam received from the prism in a first direction and a second direction, respectively; and
detecting, by a detector arrangement, the reflected p-polarization light beam and the reflected s-polarization light beam from the polarizing beam splitter;
wherein the optical arrangement comprises:
a light source configured for emitting a light beam comprising a p-polarization component and a s-polarization component; and
an initial beam splitter arranged between the light source and the prism, the initial beam splitter configured to receive the light beam from the light source and configured to separate the light beam into the p-polarization light beam and the s-polarization light beam;
wherein the light source is configured to be at a predetermined orientation for emitting the light beam comprising the p-polarization component and the s-polarization component, the predetermined orientation being 1° to less than 45° from an orientation of the light source which produces a light beam without the s-polarization component.