Recombinant yeast cell
AssigneeDsm IP Assets B.V.
InventorsIoannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paulus Petrus De Waal, Niels Lauret, Maarten Dirk Verhoeven, Jozef Petrus Johannes Schmitz
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Abstract
The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.
Claims15
A recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39;
- Rubisco) and one or more phosphoribulokinase (EC2.7.1.19;
- PRK), wherein the phosphoribulokinase is under control of a promoter, which has a PRK expression ratioanaerobic/aerobic of 2 or more, and wherein the PRK promoter comprises, in a sequence thereof, one or more of:
- NNNATTGTTNNN (SEQ ID NO:120);
- TCGTTYAG (SEQ ID NO:121);
- AAAAATTGTTGA (SEQ ID NO:122);
- or TCGTTYAG (SEQ ID N0:121) and AAAAATTGTTGA (SEQ ID NO:122).
Dependencies are inferred from the claim text.