Targeted antimicrobials and related compositions, methods and systems

An antimicrobial composition comprising

• an effective amount of a targeted antimicrobial protein for Bacillus cereus or a microorganism related thereto,
• polyamines;
• and
• a suitable vehicle,
• wherein the targeted antimicrobial protein has a catalytic domain and a binding domain, the targeted antimicrobial protein being BCZK2532 protein having SEQ ID NO:
• 3 and/or a related lysin protein having lytic activity,
• wherein the related lysin protein has at least a 40% percentage identity with the BCZK2532 protein having SEQ ID NO:
• 3 or a portion thereof, and
• wherein the related lysin protein has an arginine in a position corresponding upon alignment to amino acid residue 100 of SEQ ID NO:
• 3.

An antimicrobial composition comprising

• an effective amount of a targeted antimicrobial protein for Bacillus cereus or a microorganism related thereto,
• a suitable vehicle,
• one or more polyamines and/or polymixins,
• wherein the targeted antimicrobial protein has a catalytic domain and a binding domain, the targeted antimicrobial protein being BCZK2532 protein having SEQ ID NO:3 and/or a related lysin protein having lytic activity,
• wherein the related lysin protein has at least a 40% percentage identity with the BCZK2532 protein having SEQ ID NO:3 or a portion thereof, and
• wherein the related lysin protein has an arginine in a position corresponding upon alignment to amino acid residue 100 of SEQ ID NO:3,
• the targeted antimicrobial protein further comprising a tag.

An antimicrobial system comprising:

• an effective amount of one or more targeted antimicrobial proteins specific for Bacillus cereus or one or more related microorganisms,
• one or more polyamines and/or polymixins, and optionally
• suitable reagents, and
• an additional antimicrobial,
• wherein the one or more targeted antimicrobial proteins comprise BCZK2532 protein having a SEQ ID NO:
• 3 and/or one or more related lysin proteins having lytic activity;
• wherein the one or more related lysin proteins have at least a 40% percentage identity with the BCZK2532 protein having SEQ ID NO:
• 3 or a portion thereof, and
• wherein the one or more related lysin proteins have an arginine in a position corresponding upon alignment to amino acid residue 100 of SEQ ID NO:
• 3.

An antimicrobial system comprising:

• an effective amount of a targeted antimicrobial protein specific for Bacillus cereus or one or more related microorganisms;
• polyamines;
• suitable reagents, and
• an additional antimicrobial,
• wherein the targeted antimicrobial protein comprises BCZK2532 protein having a SEQ ID NO:
• 3 and/or one or more related lysin protein having lytic activity,
• wherein the related lysin protein has at least a 40% percentage identity with the BCZK2532 protein having SEQ ID NO:
• 3 or a portion thereof, and
• wherein the related lysin protein has an arginine in a position corresponding upon alignment to amino acid residue 100 of SEQ ID NO:
• 3.

An antimicrobial system comprising:

• an effective amount of a targeted antimicrobial protein specific for Bacillus cereus or one or more related microorganisms, the one or more targeted antimicrobial protein comprising BCZK2532 protein having a SEQ ID NO:
• 3 and/or one or more a related lysin protein having lytic activity;
• one or more polyamines and/or polymixins,
• one or more antibiotics, and
• optionally suitable reagents,
• wherein the one or more related lysin protein has at least a 90% percentage identity with the BCZK2532 protein having SEQ ID NO:
• 3 or a portion thereof, and
• wherein the one or more related lysin protein has an arginine in a position corresponding upon alignment to amino acid residue 100 of SEQ ID NO:
• 3,
• the targeted antimicrobial protein further comprising a tag.

An antimicrobial method comprising

• contacting a targeted antimicrobial BCZK2532 protein having SEQ ID NO:
• 3 and/or one or more related lysin proteins having lytic activity with a medium suitable to host Bacillus cereus or one or more related microorganisms, the contacting performed for a time and under condition to specifically kill or inhibit the growth of the one or more related microorganisms if present in the medium
• wherein the one or more related lysin proteins have at least a 40% percentage identity with the BCZK2532 protein having SEQ ID NO:3 or a portion thereof, and
• wherein the one or more related lysin proteins have an arginine in a position corresponding upon alignment to amino acid residue 100 of SEQ ID NO:3.

An antimicrobial method, comprising:

• administering to an individual in a therapeutically effective amount of a targeted antimicrobial BCZK2532 protein having a SEQ ID NO:
• 3 and/or one or more related lysin proteins having lytic activity to treat or prevent a condition associated with Bacillus cereus or one or more related microorganisms in the individual,
• wherein the one or more related lysin proteins have at least a 40% percentage identity with the BCZK2532 protein having SEQ ID NO:
• 3 or a portion thereof, and
• wherein the one or more related lysin proteins have an arginine in a position corresponding upon alignment to amino acid residue 100 of SEQ ID NO:
• 3.