Methods and compositions to maintain stem cell quiescence
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsMarco Quarta, Thomas A. Rando
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Abstract
Compositions and methods are provided for induction and maintenance of quiescence of stem cells.
Claims23
A serum-free liquid cell culture medium that provides for quiescence of stem cells during in vitro culture, the medium comprising each of components:
- elcatonin;
- MGCD-265, JNJ-7706621, Forskolin, Somatostatin, SB203580, SU5402, TGFbeta and serum replacement;
- and
- a hydrogel substrate.
A serum-free liquid culture medium that, when combined with a hydrogel substrate, provides for quiescence of human muscle stem cells during in vitro culture, the medium comprising each of components:
- elcatonin;
- Forskolin, SB203580, SU5402, TGFβ, insulin-transferrin-selenium and serum replacement.
A serum-free liquid cell culture medium that provides for quiescence of human muscle stem cells during in vitro culture, the medium comprising each of components:
- elcatonin;
- Forskolin, SB203580, SU5402, TGFβ, insulin-transferrin-selenium and serum replacement;
- and
- a hydrogel substrate at least 5 μm thick.
A serum-free liquid culture medium that, when combined with a hydrogel substrate, provides for quiescence of mouse muscle stem cells during in vitro culture, the medium comprising each of components:
- elcatonin;
- Forskolin, SB203580, SU5402, TGFβ, insulin-transferrin-selenium and serum replacement.
A serum-free liquid cell culture medium that provides for quiescence of mouse muscle stem cells during in vitro culture, the medium comprising each of components:
- elcatonin;
- MGCD-265, JNJ-7706621, Forskolin, somatostatin, SB203580, SU5402, TGFbeta and serum replacement;
- and
- a hydrogel substrate at least 5 μm thick.
Dependencies are inferred from the claim text.