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US 10,684,281 B2Granted
Reversible surface functionalization
AssigneeIllumina Cambridge Limited
InventorsXavier von Hatten, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Andrew A. Brown
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Abstract
Some embodiments described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.
Claims17
A substrate comprising a biological molecule immobilized to a surface of the substrate,
- wherein the biological molecule is covalently bonded to a host molecule comprising a hydrophobic portion and a hydrophilic portion;
- and wherein the host molecule is an optionally substituted cyclodextrin or a derivative thereof;
- wherein the surface of the substrate comprises functionalized silane covalently attached thereto, and the functionalized silane comprises a guest moiety that can form a host-guest complex with the host molecule;
- and
- wherein the biological molecule is immobilized through the host-guest complex between the functionalized silane and the host molecule.
2 more elements
Dependencies are inferred from the claim text.