Colorimetric filter paper assay for rapid monitoring of cholesterol level
AssigneeCOMSATS Institute of Information Technology
InventorsAkhtar Hayat, Muhammad Azhar Hayat Nawaz
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Abstract
A method for colorimetric detection of cholesterol in a sample is disclosed. The method includes adding beta-cyclodextrin and cholesterol to a phenolphthalein indicator solution in the presence of a phosphate buffer solution to create a solution medium and quantifying the cholesterol as a function of a complexed beta-cyclodextrin in the solution medium.
Claims19
A method for colorimetric detection of cholesterol in a sample, the method comprising:
- adding beta-cyclodextrin and cholesterol to a phenolphthalein indicator solution with a phosphate buffer solution to create a solution medium;
- and
- quantifying the cholesterol as a function of a complexed beta-cyclodextrin in the solution medium.
A method for colorimetric detection of cholesterol in a human serum sample using a filter paper based transducer platform, the method comprising the steps of:
- providing sonication time and concentration of a beta-cyclodextrin for the modification of a filter paper with the beta-cyclodextrin;
- quantifying the cholesterol based on recovery of a beta-cyclodextrin quenched phenolphthalein color on the filter paper based transducer platform;
- checking a response of cholesterol interfering compounds for a filter paper based assay;
- and
- detecting the cholesterol in the human serum sample.
A system for the colorimetric determination of cholesterol in a sample, the system comprising:
- a sample;
- a beta-cyclodextrin modified filter paper;
- a phenolphthalein indicator solution in phosphate buffer solution;
- a smart phone;
- and
- software to process images.
A method, comprising:
- adding a phenolphthalein indicator solution in a phosphate buffer solution to a modified filter paper containing beta-cyclodextrin;
- adding a sample containing cholesterol to the modified filter paper to create a finalized filter paper;
- taking an image of the finalized filter paper;
- and
- determining a total amount of cholesterol in the sample by comparing the image of the finalized filter paper to control images.
Dependencies are inferred from the claim text.