Systems and methods for single-molecule detection using nanotubes
AssigneeThe Trustees of Columbia University in the City of New York
InventorsSebastian Sorgenfrei, Kenneth L. Shepard, Chien-Yang Chiu, Colin Nuckolls, Steven Warren
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Abstract
A method for single-molecule detection is provided and uses a carbon nanotube having a probe entity attached thereto to define a first state of the carbon nanotube. The carbon nanotube is introduced to a target entity to define a second state of the carbon nanotube. The electrical conductance of the carbon nanotube in the first and second states is compared to detect the presence of a biomolecular entity. A system for single-molecule detection including a carbon nanotube is also provided.
Claims20
A method for single-molecule detection, comprising:
- providing a carbon nanotube having a cylindrical outer surface and a single-stranded molecule located in a proximity of the cylindrical outer surface that defines a first state of the carbon nanotube;
- incorporating a single nucleotide using synthesis of a double-stranded molecule from said single-stranded molecule and a polymerase, to define a second state of the carbon nanotube;
- and
- comparing the electrical conductance of the carbon nanotube in the first and second states to detect the incorporation of the single nucleotide.
A method for single-molecule detection, comprising:
- providing a carbon nanotube having a cylindrical outer surface and a single-stranded molecule attached to the cylindrical outer surface that defines a first state of the carbon nanotube;
- incorporating a single nucleotide using synthesis of a double-stranded molecule from said single-stranded molecule and a polymerase, to define a second state of the carbon nanotube;
- and
- comparing the electrical conductance of the carbon nanotube in the first and second states to detect the incorporation of the single nucleotide.
A method for single-molecule detection, comprising:
- providing a carbon nanotube having a cylindrical outer surface and a single-stranded molecule located in a proximity of the cylindrical outer surface with a polymerase bound to the single-stranded molecule that defines a first state of the carbon nanotube;
- incorporating a single nucleotide using synthesis of a double-stranded molecule from said single-stranded molecule and said polymerase, to define a second state of the carbon nanotube;
- and
- comparing the electrical conductance of the carbon nanotube in the first and second states to detect the incorporation of the single nucleotide.
A method for single-molecule detection, comprising:
- providing a carbon nanotube having a cylindrical outer surface and a single-stranded molecule attached to the cylindrical outer surface with a polymerase bound to the single-stranded molecule that defines a first state of the carbon nanotube;
- incorporating a single nucleotide using synthesis of a double-stranded molecule from said single-stranded molecule and said polymerase, to define a second state of the carbon nanotube;
- and
- comparing the electrical conductance of the carbon nanotube in the first and second states to detect the incorporation of the single nucleotide.
Dependencies are inferred from the claim text.