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AssigneeKorea University Research and Business Foundation
InventorsSung Ok Han, Young-Chul Joo, Jeong-Eun Hyeon, Seung-Kyou You
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The present invention relates to an L-cysteine-producing mutant microorganism having introduced therein genes encoding enzymes which are involved in the L-cysteine metabolic pathway, and more particularly to an L-cysteine-producing mutant microorganism having introduced therein cysE, cysK and cysR, which are genes encoding enzymes which are involved in the L-cysteine metabolic pathway, and to a method of producing L-cysteine using the mutant microorganism. According to the present invention, L-cysteine can be produced with high efficiency as a result of regulating metabolic fluxes associated with the L-cysteine metabolic pathway of the mutant microorganism and regulating a system for supplying a sulfur source essential for synthesis of L-cysteine.
A recombinant Corynebacterium glutamicum expressing cysE comprising the nucleotide sequence of SEQ ID NO:1 and encoding a serine acetyltransferase, cysK comprising the nucleotide sequence of SEQ ID NO:2 and encoding an acetylserine sulfhydrylase, and cysR comprising the nucleotide sequence of SEQ ID NO:3 and encoding a sulfur supply gene group-activating transcriptional regulator, wherein the Corynebacterium glutamicum produces L-cysteine from glucose.
Dependencies are inferred from the claim text.