Cysteine reactive probes and uses thereof
AssigneeThe Scripps Research Institute
InventorsBenjamin F. Cravatt, Keriann M. Backus, Bruno E. Correia, Megan M. Blewett, John R. Teijaro
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods, compositions, probes, polypeptides, assays, and kits for identifying a cysteine containing protein as a binding target for a small molecule fragment. Also disclosed herein are methods, compositions, and probes for mapping a biologically active cysteine site on a protein and screening a small molecule fragment for interaction with a cysteine containing protein.
Claims5
A modified pro-caspase 8 (pro-CASP8) protein comprising a non-naturally occurring small molecule fragment having a covalent bond to Cys360 of the pro-CASP8 protein, wherein:
- the pro-CASP8 protein comprises SEQ ID NO:
- 3;
- the pro-CASP8 protein has a structure of Formula (I):
- wherein:
- S is the sulfur atom of Cys360, and
- R is
- wherein R1 is H;
- and
- F′ is selected from the group consisting of
Dependencies are inferred from the claim text.