Back to results
US 10,669,575 B2Granted
Square wave thermal cycling
AssigneeBecton, Dickinson and Company
InventorAdam Steel
Life of this patent
Estimating the term…
Abstract
Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.
Claims17
A system for determining the melting temperature of a duplex nucleic acid molecule, said system comprising:
- a programmable thermal cycling apparatus;
- a detector;
- and
- a program for square wave thermal cycling, said program comprising instructions for:
- subjecting a solution comprising a double-stranded nucleic acid molecule to a square wave temperature gradient, said gradient comprising a range of temperatures that causes at least one transition of said double-stranded nucleic acid molecule to a single-stranded nucleic acid molecules;
- and
- detecting said at least one transition over the course of said square wave temperature gradient,
- wherein said square wave temperature gradient comprises a series of temperature pulses, each pulse comprising:
- a) increasing the temperature of said solution to a first high temperature, and
- b) decreasing the temperature of said solution to a second low temperature, wherein said first high temperature is greater than said second low temperature, and
- wherein, in subsequent pulses, said first high temperature and said second low temperature are each adjusted to be greater than the first and second temperatures, respectively, in prior pulses.
8 more elements
A system for determining the melting temperature of a duplex nucleic acid molecule, the system comprising:
- a programmable thermal cycling apparatus;
- a detector;
- and
- a program for square wave thermal cycling, the program comprising instructions for:
- subjecting a solution comprising single-stranded nucleic acid molecules to a square wave temperature gradient, the gradient comprising a range of temperatures that causes at least one transition of the single-stranded nucleic acid molecules to double-stranded nucleic acid molecules;
- and
- detecting the at least one transition over the course of the square wave temperature gradient,
- wherein the square wave temperature gradient comprises a series of temperature pulses, each pulse comprising:
- a) increasing the temperature of the solution to a first high temperature, and
- b) decreasing the temperature of the solution to a second low temperature, wherein the first high temperature is greater than the second low temperature, and
- wherein, in subsequent pulses, the first high temperature and the second low temperature are each adjusted to be less than the first and second temperatures, respectively, in prior pulses.
8 more elements
Dependencies are inferred from the claim text.