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US 10,669,534 B2Granted
Nanocaged enzymes with enhanced catalytic activity and increased stability
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsJinglin Fu, Zhao Zhao, Neal Woodbury, Hao Yan
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Abstract
The present disclosure describes a nanoparticle comprising a three dimensional DNA nanocage and a payload biological macromolecule, and methods of assembly thereof.
Claims19
A nanocage comprising:
- a plurality of structural members comprising M13 viral DNA, wherein internal surfaces of the plurality of structural members form an inner cavity, wherein architectural arrangement of the structural members forms a three-dimensional body having an arrangement selected from the group consisting of a honeycomb lattice, a single-walled square lattice, and a double-walled square lattice;
- and
- one or more payload molecules in said inner cavity.
A nanoparticle comprising:
- a nanocage comprising a plurality of structural members comprising DNA in a three-dimensional lattice, wherein internal surfaces of the plurality of structural members form an inner cavity;
- and
- one or more payload molecules bound to internal surfaces of the inner cavity, wherein the one or more payload molecules comprise enzymes, nucleic acids, polypeptides, antibodies, or any combination thereof,
- wherein a first structural member of the plurality of structural members is linked to a second structural member of the plurality of structural members by short bridge DNA strands,
- wherein said enzymes, polypeptides, or antibodies comprise at least one lysine residue.
2 more elements
A method of making a nanoparticle comprising:
- trapping a payload macromolecule in an open half cage comprising M13 viral DNA;
- and
- assembling two of said open half cages into a closed nanocage having an inner cavity and nanopores;
- wherein the inner cavity comprises the payload macromolecule.
1 more element
Dependencies are inferred from the claim text.