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US 10,669,351 B2Granted
Split intein compositions
AssigneeOhio State Innovation Raundation
InventorsDavid Webster Wood, Changhua Shi
Life of this patent
Estimating the term…
Abstract
Disclosed herein is a protein purification system and methods of using the system. In particular, disclosed is a split intein comprising an N-terminal intein segment, which can be immobilized, and a C-terminal intent segment, which has the property of being self-cleaving, and which can be attached to a protein of interest. Through the self-cleaving mechanism of the intein, the protein of interest can be purified.
Claims21
A split intein comprising two separate peptides:
- an N-terminal intein segment and a C-terminal intein segment, wherein the N-terminal intein segment comprises one or more amino acids at its C-terminus which allow for covalent immobilization of the N-terminal intein segment to a solid support, and wherein cleaving of the C-terminal intein segment is more sensitive to extrinsic conditions when compared to a native intein.
Dependencies are inferred from the claim text.