Engineered biosynthetic pathways for production of (6E)-8-hydroxygeraniol by fermentation

An engineered microbial cell, wherein the engineered microbial cell expresses:

• (a) a non-native geranyl diphosphate diphosphatase (geraniol synthase);
• and
• (b) a non-native geraniol-8-hydroxylase;
• wherein the engineered microbial cell comprises increased activity of one or more upstream (6E)-8-hydroxygeraniol pathway enzyme(s), said increased activity being increased relative to a control cell, the one or more upstream pathway enzymes being selected from the group consisting of ATP-citrate synthase, an acetyl-CoA synthetase, a thiolase, a hydroxymethylglutaryl coenzyme A synthase (HMG-CoA synthase), a hydroxymethylglutaryl coenzyme A reductase (HMG-CoA reductase), a mevalonate kinase, a phosphomevalonate kinase, a diphosphomevalonate decarboxylase, an isopentenyl-diphosphate delta-isomerase, and a geranyl diphosphate synthase;
• and
• wherein, when cultured, the engineered microbial cell produces (6E)-8-hydroxygeraniol at a level greater than 10 mg/L of culture medium.