# US 10,655,184 B2 · Methods and compositions involving miR-135b for distinguishing pancreatic cancer from benign pancreatic disease

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- Number: US 10,655,184 B2
- Document ID: US10655184B2
- Status: Granted
- Kind: B2
- Application number: 15/491,399
- Filed: 2017-04-19
- Published: 2018-03-08
- Granted: 2020-05-19
- Earliest claimed: 2011-09-13
- Assignees: Interpace Diagnostics Corporation
- Applicants: Interpace Diagnostics, LLC
- Inventors: Alex Adai; Anna Szafranska-Schwarzbach; Bernard Andruss; Stephan Albrecht Hahn
- Web page: https://patentgrep.com/patent/US10655184B2

## CPC

- C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575)
- C12Q 1/6844: Nucleic acid amplification reactions
- C12Q 2600/158: Expression markers
- C12Q 2600/178: miRNA, siRNA or ncRNA
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Provided are methods and compositions for identifying a miRNA profile for a particular condition, such as pancreatic disease, and using the profile in assessing the condition of a patient.

## Claims

### Claim 1

A method of producing amplified, labeled miRNA molecules from a pancreatic sample from a subject, the method comprising:
contacting the pancreatic sample from the subject with primers specific for a group of microRNAs consisting of up to 11 different labeled miRNAs that include at least miR-135b and at least 4 of the following miRNAs: miR148a, miR-130b, miR-196a, miR-24, miR-375, and miR-96,
wherein miR-217 is not one of the up to 11 different miRNAs; and
reacting the sample with the primers under conditions to amplify the labeled microRNA molecules using polymerase chain reaction to produce the amplified, labeled miRNA molecules.

### Claim 2 (depends on 1)

The method of claim 1, wherein the group of microRNAs consists of up to 7 different labeled miRNA molecules.

### Claim 3 (depends on 1)

The method of claim 1, wherein the pancreatic sample is a biopsy.

### Claim 4 (depends on 3)

The method of claim 3, wherein the biopsy is surgical resection or a fine needle aspirate.

### Claim 5 (depends on 1)

The method of claim 1, further comprising obtaining a sample from the subject.

### Claim 6 (depends on 1)

The method of claim 1, further comprising labeling the miRNA molecules from a pancreatic sample prior to reacting the sample with the primers.