Modulation of lipid metabolism for protein production
AssigneeLonza Ltd.
InventorsJames Budge, Christopher Mark Smales, Tanya Jeane Knight, Robert Young
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Abstract
The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.
Claims7
A cell comprising:
- a first exogenous nucleic acid encoding a first lipid metabolism modulator (LMM), wherein the first LMM comprises a sterol regulatory element-binding transcription factor 1 (SREBF1) or a functional fragment or isoform thereof;
- and
- an exogenous nucleic acid encoding a recombinant polypeptide,
- wherein the first exogenous nucleic acid is integrated into the chromosomal genome of the cell;
- and
- wherein the cell is a CHO-derived cell.
Dependencies are inferred from the claim text.