Methods and compositions related to lipoproteins in blood samples
AssigneeSpectracell Laboratories, Inc.
InventorsJan M. Troup, Jane E. Lines, Christina P. Tan, Kristina Henkel Holan
Life of this patent
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Abstract
Methods and compositions related to lipoproteins reactions are provided. Particularly, quantitation of lipoprotein subgroup particle numbers may be used to detect lipoprotein's response to oxidation conditions and incubation conditions. In further aspects, ultracentrifugation may be improved to analyze the lipoprotein profiles. Embodiments are directed to methods for generating a oxidation susceptibility measure for serum lipoproteins. In certain aspects a first portion of a serum sample is used to generate a base lipid particle profile (LPP) and a second portion of the serum sample is used to generate an oxidized lipid particle profile.
Claims19
A method for generating an oxidation susceptibility lipoprotein profile for a for a subject, comprising the steps of:
- (a) generating a base lipid particle profile by (i) separating a first portion of a test blood sample into very low density lipoprotein (VLDL), intermediate density lipoprotein (IDL), LDL I, LDL II, LDL III, LDL IV, high density lipoprotein (HDL) 2b, HDL 2a, and HDL 3 subgroups, (ii) measuring an amount or percentage or both of one or more of the subgroups or an amount of cholesterol in one or more subgroups;
- (b) generating an oxidized lipid particle profile by (i) oxidizing a second portion of the blood sample with an external oxidizing agent, (ii) separating the second portion of the test blood sample into VLDL, IDL, LDL I, LDL II, LDL III, LDL IV, HDL 2b, HDL 2a, and HDL 3 subgroups, (iii) measuring an amount or percentage or both of one or more of the subgroups or an amount of cholesterol in one or more subgroups;
- (c) comparing the base lipid particle profile with the oxidized lipid particle profile by comparing the measured amounts or percentage of corresponding one or more subgroups, or amounts of cholesterol in corresponding one or more subgroups to determine the oxidation susceptibility lipoprotein profile for the subject.
Dependencies are inferred from the claim text.