Quantitation of insulin-like growth factor-I and insulin-like growth factor-II with high-resolution mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsCory Bystrom, Shijun Sheng, Nigel Clarke, Richard E. Reitz
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Abstract
Methods are provided for determining the amount of an IGF-I and/or IGF-II protein in a sample using high resolution/high accuracy mass spectrometry. The methods generally comprise enriching an IGF-I and/or IGF-II protein in a sample, ionizing an IGF-I and/or IGF-II protein from the sample to generate IGF-I and/or IGF-II protein ions, and determining the amount of IGF-I and/or IGF-II protein ions with high resolution/high accuracy mass spectrometry.
Claims11
A method for determining the amount of an insulin-like growth factor-I (IGF-I) protein in a sample, the method comprising:
- a. ionizing the sample to produce one or more ions detectable by mass spectrometry;
- and
- b. determining the amount of one or more of the ions comprising an ion with a mass-to-charge ratio of 850.8±2 by high resolution/high accuracy time of flight (TOF) mass spectrometry;
- and
- c. relating the amount of the determined ion or ions to the amount of IGF-I protein in the sample.
Dependencies are inferred from the claim text.