Compounds and methods for conjugation of biomolecules

A compound of the formula:

• wherein:
• A is a carbon;
• R1, R2, R4, R5, and R6, are hydrogen;
• R3 comprises X-L-, wherein:
• X is selected from a reporter molecule, a carrier molecule, a solid support, and a reactive group, that are optionally bound to one or more additional fluorophores, wherein:
• the reporter molecule comprises a chromophore, a fluorophore, a fluorescent protein, a phosphorescent dye, a tandem dye, a particle, a hapten, an enzyme, or a radioisotope;
• the carrier molecule is an amino acid, a peptide, a protein, an antibody, an antibody fragment, an antigen, a polysaccharide, a nucleoside, a nucleotide, an oligonucleotide, a nucleic acid, a hapten, a psoralen, a hormone, a lipid, a lipid assembly, a tyramine, a synthetic polymer, a polymeric microparticle, a biological cell, a cellular compartment, an ion chelating moiety, an enzymatic substrate, or a virus;
• the solid support is an aerogel, a hydrogel, a resin, a silica gel, a bead, a biochip, a microfluidic chip, a silicon ship, a multi-well plate, a membrane, a polymeric membrane, a particle, a derivatized plastic film, a glass bead, cotton, a plastic bead, alumina gel, polysaccharide, poly(acrylate), polystyrene, poly(acrylamide), polyol, agarose, agar, cellulose, dextran, starch, heparin, glycogen, amylopectin, mannan, inulin, nitrocellulose, diazocellulose, polyvinylchloride, polypropylene, polyethylene, nylon, latex bead, a conducting metal, a nonconducting metal, glass, magnetic bead, paramagnetic bead, superparamagnetic bead, or a magnetic support;
• the reactive group is a carboxylic acid, an activated ester of carboxylic acid, an amine, a hydrazine, a haloacetamide, an alkyl halide, an isothiocynate or a maleimide group;
• and
• L is NH—(CH2)n—NH—C(O)—, wherein n is 1 to 12;
• Z is a single covalent bond;
• and
• G is an azide.