IgG subtyping assay for identifying transplantable tissue samples
AssigneeNew Jersey Organ and Tissue Sharing Network, Inc.
InventorPrakash Rao
Life of this patent
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Abstract
The disclosure relates to methods for assessing the suitability of a tissue obtained from a vertebrate (e.g., human) donor for grafting to a recipient, such as another vertebrate of the same species. The method involves contacting leukocytes obtained from the donor with serum from the recipient. Binding between recipient antibodies and the leukocytes is assessed, specifically focusing on the subtype(s) of IgG antibodies which bind with donor leukocytes. Detected binding between recipient antibodies of subtypes IgG1 and IgG3 indicates that the donor tissue is not suitable for grafting to the recipient. Detected binding between recipient antibodies of subtypes IgG2 and/or IgG4 indicates that the donor tissue can suitably be grafted to the recipient.
Claims13
A method of grafting a tissue obtained from a potential vertebrate donor to a potential recipient, the method comprising:
- inhibiting Fc receptors situated on peripheral blood mononuclear cells obtained from the potential donor from non-specific antibody binding,
- after the inhibiting step, contacting the peripheral blood mononuclear cells obtained from the potential donor with antibodies in a sample of a body fluid obtained from the potential recipient,
- contacting the antibodies of the potential recipient with differentially labeled antibodies that specifically recognize antibodies of at least IgG subtypes IgG1 and IgG3,
- detecting a degree of binding between the peripheral blood mononuclear cells of the potential donor and the antibodies of at least IgG subtypes IgG1 and IgG3 of the potential recipient in excess of a basal level of binding, and
- grafting the tissue obtained from the potential donor to the potential recipient if substantially no binding between the peripheral blood mononuclear cells of the potential donor and the antibodies of IgG subtypes IgG1 and IgG3 of the potential recipient in excess of the basal level of binding is detected,
- wherein the potential donor is a human.
Dependencies are inferred from the claim text.