Methods for detecting novel autoantibodies in Crohn's disease

A method for detecting an autoantibody of Crohn's disease in a subject comprising:

• disposing a plurality of DNA segments corresponding to different open reading frames of genes on an array, wherein each DNA segment comprises a nucleic acid sequence encoding a fusion tag;
• disposing a plurality of antibodies raised against the fusion tag on the array;
• translating the plurality of DNA segments to a plurality of polypeptides, wherein each polypeptide comprises the fusion tag and is captured by the antibodies raised against the fusion tag, wherein the plurality of captured polypeptides are selected from the group consisting of A4-Fla2, flaD, XAGE2, CDC42EP1, PPP1R13L, FOSL1, SNRPB, CCHCR1, LCORL, NUPL1, PRPH, PTTG1, SNAII and ORC2L, and incubating the array comprising the plurality of captured polypeptides with a sample collected from a subject suspecting having the disease, identifying the subject having Crohn's disease based, at least in part, on the presence of the polypeptide/autoantibody complex;
• and
• administering a medical therapy selected from the group consisting of therapeutic steroids, immunomodulators, and biologics.