Single channel chemiluminescent micro-fluidic chip and detection method thereof

A single-channel chemiluminescent micro-fluidic chip, including a chip body, wherein the chip body includes an optical path scanning window, a whole blood filter cavity, a quantification-reaction cavity and a waste liquid cavity, a fluid outlet of the whole blood filter cavity is sequentially communicated with the quantification-reaction cavity and the waste liquid cavity through a micro-fluidic passage, the optical path scanning window is disposed over the quantification-reaction cavity, a fluid inlet of the quantification-reaction cavity is communicated with external fluid paths through a cleaning solution delivery branch and a chromogenic reagent delivery branch respectively, and the whole blood filter cavity is communicated with an external air path;

• characterized in that the quantification-reaction cavity is composed of a quantification-reaction pool on a lower portion and a reaction pool cover plate for sealing an opening of the quantification-reaction pool, the quantification-reaction pool is equally divided into three cavities by two partition plates a disposed perpendicular to a fluid flow direction, a labeled antibody is placed in the middle cavity, coated antibodies are placed in the partitioned cavities on two sides, a surface, facing the quantification-reaction pool, of the reaction pool cover plate is equally divided into two cover plate parts by a partition plate b disposed perpendicular to the fluid flow direction, and the coated antibodies are embedded in the cover plate parts.