In vitro test system to evaluate xenobiotics as immune-modulators of drug transport and metabolism in human hepatocytes
AssigneeXenotech LLC
InventorsMaciej Czerwinski, David Benjamin Buckley, Faraz Kazmi
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Abstract
A method for evaluating in vitro the effect of a xenobiotic (e.g., a drug) on drug metabolism in hepatocytes. The kit comprises a first culture of hepatocytes, a portion of in vitro xenobiotic-stimulated biological sample, and instructions for incubating the first culture of hepatocytes with the portion of in vitro xenobiotic-stimulated biological sample, and analyzing the activity, expression, or a combination thereof of a biomarker in the hepatocytes to evaluate the effect of the xenobiotic on drug metabolism in the hepatocytes.
Claims20
A in vitro method of evaluating the effect of a xenobiotic on drug metabolism in hepatocytes, wherein said xenobiotic is a small molecule drug, said method comprising:
- providing a first culture of hepatocytes;
- providing a portion of an in vitro xenobiotic-stimulated biological sample by:
- providing a first culture of a biological sample;
- exposing said first biological sample to said xenobiotic small molecule drug in vitro for a period of time to yield said in vitro xenobiotic-stimulated biological sample;
- and
- separating said portion of said in vitro xenobiotic-stimulated biological sample;
- transferring said portion of said in vitro xenobiotic-stimulated biological sample to said first culture of hepatocytes;
- and
- detecting the activity, expression, or combination thereof of a biomarker in said hepatocytes to thereby evaluate the effect of said in vitro xenobiotic on said drug metabolism in said hepatocytes.
Dependencies are inferred from the claim text.