Lipid bilayer-integrated SPP1 connector protein nanopore and SPP1 connector protein variants for use as lipid bilayer-integrated nanopore
AssigneeUniversity of Kentucky Research Foundation
InventorsPeixuan Guo, Shaoying Wang
Life of this patent
Estimating the term…
Abstract
A conductive channel-containing membrane includes a membrane layer, and a SPP1 connector polypeptide variant that is incorporated into the membrane layer to form an aperture through which conductance can occur when an electrical potential is applied across the membrane. A method of sensing a molecule, such as a polypeptide or nucleic acid molecule, makes use of the conductive channel-containing membrane. A method of DNA sequence makes use of the conductive channel-containing membrane.
Claims14
A nucleic acid molecule encoding a SPP1 connector polypeptide variant, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:6, SEQ ID NO:8, or SEQ ID NO:10.
A SPP1 connector polypeptide variant, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:6, SEQ ID NO:8, or SEQ ID NO:10.
A SPP1 connector polypeptide variant, wherein the polypeptide comprises an amino acid sequence encoded by a nucleic acid molecule having the sequence of SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:9.
A conductive channel-containing membrane, comprising:
- (a) a membrane layer;
- and
- (b) a SPP1 connector polypeptide variant that is incorporated into the membrane layer to form an aperture through which conductance can occur when an electrical potential is applied across the membrane, wherein the SPP1 connector polypeptide variant is selected from:
- a polypeptide comprising the amino acid sequence of SEQ ID NO:6, SEQ ID NO:8, or SEQ ID NO:10;
- and
- a polypeptide comprising an amino acid sequence encoded by a nucleic acid molecule having the sequence of SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:9.
Dependencies are inferred from the claim text.