Liquid chromatograph method, information provision method, and liquid chromatographic auxiliary apparatus

An information provision method in liquid chromatography for separating a mixture of two or more kinds of compounds, comprising the following steps:

• (1) performing at least two thin-layer chromatography or column chromatography steps wherein each of the at least two of said steps is performed for a different mixing ratio from the other;
• (2) creating a relational expression between a mixing ratio and an elution degree of a solvent for each compound in a sample based on results of the step (1);
• and
• (3) providing information concerning chromatography based on the relational expression,
• wherein the step (3) is performed in a liquid chromatography condition wherein Rs as defined below is more than 1.2,
• wherein Rs=[N1/2/4][(α−1)/α][k′/(k′+1],
• N represent a theoretical plate number of a column,
• α=k′1/k′2 and
• k′1 and k′2 represent relative retention for compounds 1 and 2, respectively.

A liquid chromatographic auxiliary apparatus, comprising:

• a calculation formula creation part creating a relationship between a mixing ratio and an elution degree of a solvent for each compound in a sample based on a result of at least two thin-layer chromatography or column chromatography steps wherein each of the at least two of said steps is performed for a different mixing ratio from the other;
• and
• a liquid chromatography result prediction part determining an optimum condition of liquid chromatography based on results of the calculation formula creation part,
• wherein the optimum condition of the liquid chromatography condition is the one where Rs as defined below is more than 1.2,
• wherein Rs=[N1/2/4][(α−1)/α][k′/(k′+1],
• N represent a theoretical plate number of a column,
• α=k′1/k′2 and
• k′1 and k′2 represent relative retention for compounds 1 and 2, respectively.