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US 10,648,913 B2Granted
System and method for detecting multiple-excitation-induced light in a flow channel
AssigneeACEA Biosciences, Inc.
InventorsNan Li, Jian Wu, Ye Chen, Tianxing Wang, Xiaobo Wang
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Abstract
A system for detecting signal components of light induced by multiple excitation sources, which includes a flow channel having two spatially separated optical interrogation zones; a light illumination subsystem that directs each of two light beams of different wavelengths to a different zone of the optical interrogation zones; a detector subsystem that converts detected light into a total electrical signal having both modulated and unmodulated electrical signals; and a processor that determines signal components from the light detected from each of the optical interrogation zones.
Claims16
A system for detecting signal components of light induced by multiple excitation sources, the system comprising:
- a flow channel configured for the flow of particles, the flow channel comprising a first optical interrogation zone and a second optical interrogation zone, wherein the first optical interrogation zone and the second optical interrogation zone are spatially separated;
- a light illumination subsystem configured to direct a first light beam to the first optical interrogation zone and to direct a second beam to the second optical interrogation zone, wherein the wavelengths of the first light beam are different from the wavelengths of the second light beam, wherein the first light beam is intensity modulated at a modulating frequency that is a constant pattern repeating over time and the second light beam is not intensity modulated to provide a constant intensity;
- shared light collection optics configured to collect light from both of the optical interrogation zones, wherein the light comprises a first portion of light from the first interrogation zone and a second portion of light from the second interrogation zone, the first portion of light being intensity modulated at the modulating frequency and resulting from the first beam being directed to the first optical interrogation zone;
- shared light splitting optics configured to split the collected light into different channels, wherein the shared light splitting optics comprise a filter shared by each of the optical interrogation zones;
- a detector subsystem comprising a set of at least two light detectors and configured to receive the channeled light, detect changes in the channeled light in response to particles flowing through the optical interrogation zones, and to convert the detected light into a total electrical signal proportional to detected modulated and unmodulated light;
- and
- a processor configured to receive the total electrical signal from the detector subsystem, to de-modulate the portion of the total electrical signal proportional to the modulated light, and to determine signal components from the light detected from each of the optical interrogation zones.
4 more elements
A method of detecting signal components from light induced by multiple excitation sources, the method comprising:
- providing a flow channel comprising a first optical interrogation zone and a second optical interrogation zone, wherein the first and second optical interrogation zones are spatially separated;
- flowing a population of particles labeled with at least two different fluorescent molecules through each of the first and second optical interrogation zones, wherein the at least two different fluorescent molecules comprise a first fluorescent molecule and a second fluorescent molecule;
- directing at a constant intensity and to the first optical interrogation zones, a first light beam comprising a wavelength that induces fluorescence of the first fluorescent molecules;
- directing at a modulated intensity and to the second optical interrogation zones, a second light beam comprising a wavelength that induces fluorescence of the first or second fluorescent molecule, wherein the modulated intensity is a constant pattern repeating over time;
- collecting light from both of the optical interrogation zones with shared collection optics, wherein the light comprises a first portion of light from the first interrogation zone and a second portion of light from the second interrogation zone, the intensity of the second portion of light being modulated at the constant pattern and resulting from the second beam being directed to the second optical interrogation zone;
- splitting the collected light into different channels using a filter shared by the light collected from each of the optical interrogation zones;
- detecting the split light;
- converting the detected light into a total electrical signal proportional to detected modulated and unmodulated light;
- de-modulating the portion of the total electrical signal proportional to the modulated light;
- and
- determining signal components of the light detected from each of the optical interrogation zones.
8 more elements
Dependencies are inferred from the claim text.