Use of methylation sites in Y chromosome as prostate cancer diagnosis marker
AssigneeWuxi Shenrui Bio-Pharmaceuticals Co., Ltd.
InventorsYingli Sun, Lushuai Yao
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Abstract
Disclosed is a use of methylation sites as a disease diagnosis marker, in particular relating to a use of methylation sites in a Y chromosome as a prostate cancer diagnosis marker. More particularly, the method and criterion for screening methylation sites in chromosomes associated with a disease are established in the present invention, and using prostate cancer as an example, six methylation sites in the Y chromosome associated with the prostate cancer diagnosis are screened out. Methylation sites in the Y chromosome screened can be used for early and rapid diagnosis of the disease as a prostate cancer diagnosis marker.
Claims5
A method for detecting a methylation level of a methylation site in a Y chromosome in a sample obtained from a subject, wherein the methylation site is cg05163709, said method comprising obtaining methylation levels of a test sample by pyrosequencing, wherein the pyrosequencing employs a primer pair consisting of SEQ ID NO:
- 1 and SEQ ID NO:
- 2 and a sequencing primer consisting of SEQ ID NO:
- 3, the method being useful for diagnosis for prostate cancer, assessment of a risk for prostate cancer, evaluation of a therapeutic effect on prostate cancer, and screening of a therapeutic drug for prostate cancer.
Dependencies are inferred from the claim text.