Method for the detection of multiple target nucleic acids using clamping probes and detection probes
AssigneePanagene Inc.
InventorsGoon Ho Joe, Yong Tae Kim, Sung Kee Kim, Jin Woo Kim, Jae Jin Choi, In Seong Wo, Ji Hye Yoon, Su Nam Kim, Jun Ho Park
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Abstract
Disclosed is a probe mixture for real-time detection of target nucleic acids comprising at least one detection probe and at least one clamping probe for inhibiting amplification of wild type genes or unwanted genes, a kit using the same and a method for real-time detection of target nucleic acids using the mixture and the kit.
Claims24
A probe mixture for real-time detection and simultaneous multiple detection of target nucleic acids comprising at least one detection probe and at least one clamping probe for inhibiting amplification of wild type genes or unwanted genes where an amino acid or a side chain of the amino acid is attached to the detection probe or clamping probe to structurally modify the probe such that a mutation can be detected with 0.01% sensitivity by simultaneously analyzing a real-time amplification curve and a melting curve,
- wherein the amino acid is selected from the group consisting L-glutamic acid, and D-glutamic acid.
A method for simultaneous detection of multiple target nucleic acids using a probe mixture for real-time detection of target nucleic acids comprising at least one detection probe and at least one clamping probe for inhibiting amplification of wild type genes or unwanted genes where an amino acid or a side chain of the amino acid is attached to the detection probe or clamping probe to structurally modify the probe comprising detecting a mutation with 0.01% sensitivity by simultaneously analyzing a real-time amplification curve and a melting curve, wherein the amino acid is selected from the group consisting of L-glutamic acid, and D-glutamic acid.
Dependencies are inferred from the claim text.