Compositions and methods to control insect pests
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsXu Hu, Bliss Marie Kernodle, Molly Mary McMahon, Adane Kassa, Xiping Niu, James Kevin Presnail, Albert L. Lu, Nina Richtman, Jonathan William Robeson
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Abstract
The present invention relates generally to methods of molecular biology and gene silencing to control pests.
Claims33
A silencing element comprising at least one double-stranded RNA region, at least one strand of which comprises a polynucleotide that is complementary to:
- (a) the nucleotide sequence comprising SEQ ID NO:
- 135;
- (b) the nucleotide sequence comprising at least 90% sequence identity to SEQ ID NO:
- 135;
- or
- (c) the nucleotide sequence comprising at least 25 consecutive nucleotides of SEQ ID NO:
- 135;
- wherein the silencing element has sterilization activity against an insect plant pest.
A plant cell having stably incorporated into its genome a heterologous polynucleotide encoding a silencing element, wherein the polynucleotide comprises:
- a. the nucleotide sequence comprising SEQ ID NO:
- 135;
- b. the nucleotide sequence comprising at least 90% sequence identity to SEQ ID NO:
- 135;
- or
- c. the nucleotide sequence comprising at least 25 consecutive nucleotides of SEQ ID NO:
- 135;
- wherein the silencing element decreases the fertility of an insect plant pest.
A method for controlling a plant insect pest comprising feeding to a plant insect pest a composition comprising a silencing element, wherein the silencing element controls the plant pest, wherein the silencing element comprises a sequence complementary to:
- a. the nucleotide sequence comprising SEQ ID NO:
- 135;
- b. the nucleotide sequence comprising at least 90% sequence identity to SEQ ID NO:
- 135;
- or
- the nucleotide sequence comprising at least 25 consecutive nucleotides of SEQ ID NO:
- 135;
- wherein the silencing element has insect sterilization activity against the plant pest.
Dependencies are inferred from the claim text.