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AssigneeEmpresa Brasileira De Pesquisa AgropecuáRia—Embrapa
InventorsMaria Fatima Grossi De Sa, Gustavo Ramos De Oliveira, Maria Cristina Mattar Da Silva, Thales Lima Rocha, Mariana Torquato Quezado De Magalhaes
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The present invention belongs to an insecticide compound derived from a strain of Bacillus thuringiensis and refers to the field of controlling plant pests, particularly to the control of the boll weevil—Anthonomus grandis. More specifically, the object of the invention refers to a new gene for the new delta-endotoxin designated Cry8Ha and to the cloning and expression of the gene that encodes for the protein Cry8Ha in Escherichia coli. There is provided the nucleotide sequence and encoding protein of the new delta-endotoxin, recombinant vectors and host cells. There is also provided processes and means for recombinant production and the use of the new delta-endotoxin for application in the control of the boll weevil. Additionally, the invention also provides an optimized synthetic gene for expression in cotton plants. Using the gene described herein, it is possible to transform plants based on techniques known by specialists in the art, for the expression of the endotoxin active against the boll weevil.
A nucleic acid molecule, wherein said nucleic acid molecule comprises a polynucleotide sequence encoding a protein having at least 95% identity to SEQ ID NO:
An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
A genic construct comprising:
A transgenic cell containing an optimized polynucleotide sequence for expression in plants, wherein the polynucleotide comprises the sequence of SEQ ID NO:
Dependencies are inferred from the claim text.