Composition and methods for producing tobacco plants and products having reduced tobacco-specific nitrosamines (TSNAs)
AssigneeAltria Client Services LLC
InventorsChengalrayan Kudithipudi, Dongmei Xu, James Strickland
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Abstract
The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radical absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.
Claims26
A method for reducing the level of one or more tobacco-specific nitrosamines (TSNAs) in cured tobacco leaf or a tobacco product made therefrom, said method comprising
- a) increasing the level of one or more antioxidants in a tobacco plant via a transgene encoding a protein comprising a sequence having at least 90% identity to a sequence selected from the group consisting of SEQ ID No. 13 and 23;
- and
- b) reducing the level of said one or more TSNAs in cured tobacco leaf from said tobacco plant or a tobacco product made from said cured tobacco leaf.
A method for manufacturing a tobacco product having a reduced level of one or more TSNAs, said method comprising
- a) obtaining cured tobacco leaf comprising a transgene encoding a protein comprising a sequence having at least 90% identity to a sequence selected from the group consisting of SEQ ID No. 13 and 23, wherein the transgene provides an increased level of one or more antioxidants in said cured tobacco;
- and
- b) manufacturing a tobacco product comprising a reduced level of said one or more TSNAs using said cured tobacco leaf.
Dependencies are inferred from the claim text.