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US 10,647,982 B1Granted
Automated cell processing methods, modules, instruments, and systems
AssigneeInscripta, Inc.
InventorsDon Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
Life of this patent
Estimating the term…
Abstract
In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
Claims20
An automated stand-alone multi-module cell editing instrument for performing nuclease editing comprising:
- a receptacle configured to receive cells and/or nucleic acids;
- a growth module for growing the cells for transformation;
- a cell concentration module to concentrate the cells and render the cells electrocompetent;
- a transformation module configured to introduce nucleic acids into the cells;
- a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells;
- a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script, wherein the processor comprises a robotics controller, module controller, temperature controller, and a CPU;
- and
- an automated liquid handling system to move liquids from the receptacle to the growth module, from the growth module to the cell concentration module, from the cell concentration module to the transformation module, from the transformation module to the nuclease-directed editing module, without user intervention.
5 more elements
An automated stand-alone multi-module cell editing instrument for performing nuclease editing comprising:
- a receptacle configured to receive cells/or nucleic acids;
- a transformation module comprising an electroporator to introduce the nucleic acids into the cells;
- a selection module;
- a nuclease-directed editing module configured to select for transformed cells and to allow the nucleic acids to edit nucleic acids in the cells to produce edited cells;
- a processing environment comprising a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a script, wherein the processor comprises a robotics controller, module controller and temperature controller;
- and
- an automated liquid handling system to move liquids from the receptacle to the transformation module, from the transformation module to the selection module, and from the selection module to the nuclease-directed editing module, all without user intervention.
4 more elements
Dependencies are inferred from the claim text.