Modified heterocyclase
AssigneesThe University Court of the University of Aberdeen, University Court of the University of St Andrews
InventorsJames Naismith, Jesko Koehnke, Andrew Bent, Nicholas Westwood, Greg Mann, Wael Houssen Ibrahim, Marcel Jaspars, Ying Ge
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Abstract
This invention relates to an engineered leader-independent heterocyclase (also known as a cyclodehydratase) comprising a defined cyanobactin leader sequence which drives the efficient conversion of heterocyclisable amino acids, such as Ser, Thr and Cys, within a peptide substrate lacking a leader sequence into heterocycles produce a homogenous heterocycle-containing product. This may be useful in biotechnology and chemical synthesis.
Claims17
A modified heterocyclase, wherein the N-terminus of the modified heterocyclase consists of a cyanobactin leader peptide selected from the group consisting of SEQ ID NO:
- 29 and SEQ ID NO:
- 30, and wherein the C-terminus of said modified heterocyclase comprises a YcaO-domain cyanobactin heterocyclase such that the modified heterocyclase is capable of introducing heterocyclic groups into a substrate that lacks a leader peptide.
Dependencies are inferred from the claim text.