Methods for obtaining positive transformants of a filamentous fungal host cell
AssigneeNovozymes, Inc.
InventorDonna Moyer
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Abstract
The present invention relates to methods for obtaining positive transformants of a filamentous fungal host cell, comprising: transforming a tandem construct into a population of cells of the filamentous fungal host a tandem construct and isolating a transformant of the filamentous fungal host cell comprising the tandem construct. The present invention also relates to such tandem constructs, filamentous fungal host cells comprising such tandem constructs, and methods of producing multiple recombinant proteins.
Claims13
A tandem construct comprising (i) one or more selectable markers, (ii) a first polynucleotide encoding a first polypeptide having biological activity operably linked to a first promoter and a first terminator, (iii) a second polynucleotide encoding a second polypeptide having biological activity operably linked to a second promoter and a second terminator, and (iv) a first homologous repeat flanking 5′ of the one or more selectable markers and a second homologous repeat flanking 3′ of the one or more selectable markers, wherein the first homologous repeat and the second homologous repeat undergo homologous recombination to excise the one or more selectable markers, wherein the tandem construct integrates by ectopic integration into the chromosome of a filamentous fungal host cell.
Dependencies are inferred from the claim text.