L-type amylase variant and use thereof
AssigneeNanjing Bestzyme Bio-Engineering Co., Ltd.
InventorsYan Fan, Yan Sun, Hong Xu
Life of this patent
Estimating the term…
Abstract
The present invention discloses an L-type amylase variant and use thereof. The α-amylase variant is obtained by deleting the first N-terminal amino acid residue V from the α-amylase of B. licheniformis and replacing it with three other amino acid residues DGL. The α-amylase variant provided by the present invention has high catalytic activity under the acidic conditions of pH 5.0-5.8 and a high temperature of 100° C. or above. The acid resistance and thermal stability of these α-amylase variants are suitable for starch liquefaction.
Claims9
An α-amylase variant, comprising at least 95% identity to parental α-amylase of SEQ ID NO:
- 2, wherein the α-amylase variant comprises amino acid residues DGL at N-terminus in place of N-terminal amino acid residue V of the parental α-amylase, and wherein the α-amylase variant still retains the ability of the parental α-amylase to hydrolyze α-1,4 glycosidic bonds.
Dependencies are inferred from the claim text.