Surface treatment for cell culture
AssigneeThe Florida State University Research Foundation, Inc.
InventorsJoseph B. Schlenoff, Carlos Arias
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Abstract
An article suitable for inducing quasispherical cell clustering is provided. The article comprising: a layer suitable for culturing quasispherical cell clusters, the layer comprising a bulk region comprising an interpenetrating network of at least one predominantly positively charged polyelectrolyte and at least one predominantly negatively charged positive polyelectrolyte, a back surface region, and a front surface region, wherein the front surface region comprises a net negative fixed surface charge density of between about 0.4 micromole per m2 and about 1.5 micromole per m2.
Claims22
An article comprising:
- a layer suitable for culturing quasispherical cell clusters, the layer comprising a bulk region comprising an interpenetrating network of at least one predominantly positively charged polyelectrolyte and at least one predominantly negatively charged polyelectrolyte, a back surface region, and a front surface region, wherein the front surface region comprises a net negative fixed surface charge density of between 0.5 micromole per m2 and 1.5 micromole per m2,
- wherein the net negative fixed surface charge density is comprised of a deposited negatively charged polyelectrolyte that is obtained by mixing a negatively charged polyelectrolyte with a conditioning salt, wherein the conditioning salt is selected from the group consisting of chloride salts, citrate salts, and phosphate salts.
Dependencies are inferred from the claim text.