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US 10,647,749 B2Granted
Glycosylated YGHJ polypeptides from enterotoxigenic Escherichia coli (ETEC)
AssigneesSyddansk Universitet, Aarhus Universitet
InventorsAnders Boysen, Jakob Møller-Jensen, Giuseppe Palmisano, Martin Røssel Larsen
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Abstract
The present invention relates to glycosylated YghJ polypeptides from or derived from enterotoxigenic Escherichia coli (ETEC) that are immunogenic. In particular, the present invention relates to compositions or vaccines comprising the polypeptides and their application in immunization, vaccination, treatment and diagnosis of ETEC.
Claims16
A method for identifying and/or mapping O-linked glycoproteins, the method comprising the following steps:
- i) provision of a sample comprising an O-linked glycoprotein,
- ii) substitution of any O-linked carbohydrate moieties with a 2-Aminoethyl phosphonic acid (AEP) group,
- iii) TiO2 enrichment of the 2-AEP-tagged glycoprotein, and
- iv) mass spectrometry of the enriched 2-AEP-tagged glycoprotein.
1 more element
Dependencies are inferred from the claim text.