Xylene-based amphiphilic compound and use thereof

A method for preparing the compound represented by Formula 1 below, the method comprising:

• 1) introducing an alkyl group by performing monoalkylation on diethyl malonate;
• 2) introducing a xylene linker by coupling the product of step 1) with bis(bromomethyl)benzene;
• 3) reducing an ester functional group of the product of step 2) into an alcohol functional group;
• 4) introducing a protective group-attached saccharide by performing glycosylation on the product of step 3);
• and
• 5) performing deprotection on the product of step 4):
• where the position of A2, relative to A1, is ortho, meta or para;
• A1 and A2 are the same or different and each independently represented by Formula 2 below;
• R1 is a substituted or unsubstituted C3-C26 alkyl group, a substituted or unsubstituted C3-C26 cycloalkyl group, or a substituted or unsubstituted C3-C26 aryl group;
• X1 and X2 are each independently a saccharide linked by an oxygen atom;
• and
• the symbol * in Formula 2 may represent a part linked to the core structure of Formula 1.

A method for extracting, solubilizing, stabilizing, crystallizing or analyzing a membrane protein, the method comprising:

• treating a membrane protein with the compound represented by Formula 1 below in an aqueous solution:
• where the position of A2, relative to A1, is ortho, meta or para;
• A1 and A2 are the same or different and each independently represented by Formula 2 below;
• R1 is a substituted or unsubstituted C3-C26 alkyl group, a substituted or unsubstituted C3-C26 cycloalkyl group, or a substituted or unsubstituted C3-C26 aryl group;
• X1 and X2 are each independently a saccharide linked by an oxygen atom;
• and
• the symbol * in Formula 2 may represent a part linked to the core structure of Formula 1.