Apparatus and methods for integrated sample preparation, reaction and detection

An apparatus, comprising:

• an isolation module comprising:
• a first housing and a second housing, the first housing defining a first chamber and a second chamber, wherein:
• at least the first chamber is configured to contain a sample;
• the second housing comprises a side wall and a puncturable member, the side wall and the puncturable member collectively defining a first volume configured to contain a first substance;
• and
• a portion of the second housing is configured to be disposed within the first housing such that the first volume is in fluid communication with the first chamber when a portion of the puncturable member is punctured;
• a reaction module defining a reaction chamber configured to contain a second substance, the reaction module configured to be coupled to the isolation module such that the reaction chamber is in fluid communication with the second chamber of the first housing;
• and
• a transfer mechanism configured to transfer one or more substances from the isolation module to the reaction module.