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AssigneeBerkeley Lights, Inc.
InventorsMark P. White, Eric D. Hobbs, J. Tanner Nevill, Daniele Malleo, Steven W. Short
Estimating the term…
A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
A process of analyzing a biological micro-object in a microfluidic device, said device comprising a microfluidic channel to which at least one microfluidic sequestration pen is fluidically connected, said at least one sequestration pen comprising a fluidic isolation structure comprising an isolation region having a single opening and a connection region fluidically connecting said isolation region to said channel, the connection region comprising a proximal opening into the channel, said process comprising:
Dependencies are inferred from the claim text.