Flow cell device for single cell analysis, and single cell analysis device

A flow cell device for single cell analysis, comprising:

• a plurality of reaction chambers each including one or more cell capturing units each configured to capture a single cell and one or more nucleic acid capturing units connected in a one-on-one relationship to the one or more cell capturing units via respective flow channels;
• a plurality of first liquid flow channels that are connected to the plurality of reaction chambers in a one-on-one relationship on a first surface side;
• and
• a second liquid flow channel that is commonly connected to the plurality of reaction chambers on a second surface side opposite to the first surface,
• wherein each of the nucleic acid capturing units has a porous structure or a structure in which a plurality of beads are packed,
• wherein each of the nucleic acid capturing units has immobilized therein one or more mRNA-capturing probes each including a DNA sequence for identifying the respective nucleic acid capturing unit thereof, and
• wherein each of the cell capturing units has an opening with a first diameter configured to capture a single cell, and each of the nucleic acid capturing units includes the porous structure or the structure in which the plurality of beads are packed and having a second diameter larger than the first diameter.

A single cell analysis apparatus, comprising:

• a stage on which a flow cell device for single cell analysis is placed, the flow cell device including:
• a plurality of reaction chambers each including one or more cell capturing units each configured to capture a single cell and one or more nucleic acid capturing units connected in a one-on-one relationship to the one or more cell capturing units via respective flow channels,
• a plurality first liquid flow channels connected to the plurality of reaction chambers in a one-on-one relationship on a first surface side, and
• a second liquid flow channel commonly connected to the plurality of reaction chambers on a second surface side opposite to the first surface;
• a stage controlling system configured to control a position of the stage;
• a first introduction controller configured to individually control introduction of a first liquid into the plurality of first fluid flow channels;
• and
• a discharge controller configured to control discharge of a second liquid from the second fluid flow channel,
• wherein each of the nucleic acid capturing units has a porous structure or a structure in which a plurality of beads are packed, and
• wherein each of the nucleic acid capturing units has immobilized therein one or more mRNA-capturing probes each including a DNA sequence for identifying the respective nucleic acid capturing unit thereof, and
• wherein each of the cell capturing units has an opening with a first diameter configured to capture a single cell, and each of the nucleic acid capturing units includes the porous structure or the structure in which the plurality of beads are packed and having a second diameter larger than the first diameter.