Multiepitope fusion antigens and vaccines and their use in treatment of enterotoxigenic diarrhea
AssigneesThe John Hopkins University, South Dakota State University
InventorsDavid A. Sack, Weiping Zhang
Life of this patent
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Abstract
Provided herein are polypeptides comprising up to 9 antigenic elements of ETEC virulence determinants: 7 CFA adhesins [CFA/I, CFA/II (CS1, CS2, CS3), CFA/IV (CS4, CS5, CS6)] expressed by the most prevalent and virulent ETEC strains, and 2 toxins expressed by all ETEC strains, were genetically fused together for CFA-toxoid fusion with proteins (CFA/I/II/IV-STa-toxoidLTtoxoid). Methods for making these polypeptides and their use in the treatment of ETEC related disease are also provided.
Claims6
A fusion polypeptide molecule which comprises the colonization factor antigen (CFA) antigenic epitopes of CFA/I (SEQ ID NO:
- 4), CFA/II (CS1 (SEQ ID NO:
- 5), CS2 (SEQ ID NO:
- 6), CS3 (SEQ ID NO:
- 7), CFA/IV (CS4 (SEQ ID NO:
- 8), CS5 (SEQ ID NO:
- 9), and CS6 (SEQ ID NO:
- 10), and wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
- 1, or having at least 90% sequence identity to SEQ ID NO:
- 1.
A method for developing an immune response to colonization factor antigen (CFA) antigenic epitopes of CFA/I (SEQ ID NO:
- 4), CFA/II (CS1 (SEQ ID NO:
- 5), CS2 (SEQ ID NO:
- 6), CS3 (SEQ ID NO:
- 7), CFA/IV (CS4 (SEQ ID NO:
- 8), CS5 (SEQ ID NO:
- 9), and CS6 (SEQ ID NO:
- 10) in a subject comprising administering to the subject an effective amount of a polypeptide molecule consisting of the amino acid sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.