Apoptotic bodies
AssigneesAlfred E. Mann Institute for Biomedical Engineering at the University of Southern California, University of Southern California
InventorsSongtao Shi, Dawei Liu, Cunye Qu
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Abstract
This disclosure relates to apoptotic bodies. The disclosure particularly relates to a composition comprising the apoptotic bodies. The disclosure further relates to preparation of apoptotic bodies from stem cells. The disclosure also relates to medical treatments comprising the use of the composition comprising the apoptotic bodies. The apoptotic bodies may comprise apoptotic stem cells. The apoptosis of a cell may be induced by a starvation method, an ultra-violet irradiation method, a thermal stress method, a staurosporine method, or a combination thereof.
Claims28
A composition comprising about 1×101 apoptotic bodies to about 1×1010 apoptotic bodies and a carrier, wherein the apoptotic bodies comprise apoptotic mesenchymal stem cells, and wherein the apoptotic mesenchymal stem cells are:
- (i) positive for expression of thrombospondin-1 (TSP-1) and positive for staining by 7-amino-actinomycin D (7-AAD);
- (ii) positive for nucleic acid staining by a dye selected from the group consisting of 1,5-bis{[2-(di-methylamino)ethyl]amino}-4,8-dihydroxyanthracene-9,10-dione (DRAQ5) and 7-AAD;
- (iii) positive for staining by DRAQ5, Annexin V and 7-AAD;
- (iv) positive for staining by 7-AAD and Annexin V;
- and
- (v) positive for expression of TSP-1 and positive for staining by Annexin V and 7-AAD.
Dependencies are inferred from the claim text.