DNA-functionalized scaffolds for drug capture applications
AssigneeCalifornia Institute of Technology
InventorsMichael D. Schulz, Carl M. Blumenfeld, Robert H. Grubbs, Julia R. Greer, Daryl Wei Liang Yee
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Abstract
This disclosure is directed to structured compositions, including DNA-functionalized scaffolds, for drug capture, and methods and devices for sequestering chemotherapeutics from physiological fluids using the functionalized scaffolds.
Claims57
A structured composition comprising DNA operatively linked to a scaffold through an organic polymer surface and/or a hydroxylated inorganic surface of the scaffold, wherein the DNA is operatively linked to the organic polymer surface or hydroxylated inorganic surface by attachment to a linking group, wherein:
- (a) the organic polymer surface comprises a lithographically patterned organic polymer;
- or
- (b) the scaffold comprises a three-dimensionally (3D) photopolymerized patterned polymer structure;
- or
- (c) the linking group is covalently bound to the organic polymer surface, the covalent bond resulting from the co-polymerization of an unsaturated moiety of a linker group precursor with a prepolymer of the organic polymer to form the linking group pendant to the surface;
- or
- (d) the organic polymer surface comprises a polyhydroxylated organic polymer comprising (co)polymerized polyvinyl alcohol, polyalkylhydroxy(meth)acrylate, polyhydroxyalkyl(meth)acrylamide, monoglycerol(meth)acrylate, monoglycerol(meth)acrylamide, or a combination thereof, or (co)polymerized (meth)acrylate linkages;
- or
- (e) the linking group is covalently bound to the hydroxylated inorganic surface.
A method of removing drugs from a physiological fluid, the method comprising contacting the physiological fluid with a structured composition for a time sufficient to remove at least a portion of the drug from the physiological fluid, wherein the structured composition comprises DNA operatively linked to a scaffold through an organic polymer surface and/or hydroxylated inorganic surface of the scaffold, wherein the DNA is linked to the organic polymer surface or hydroxylated inorganic surface by attachment to a linking group, wherein:
- (a) the organic polymer surface comprises a lithographically patterned organic polymer;
- or
- (b) the scaffold comprises a three-dimensionally (3D) photopolymerized patterned polymer structure;
- or
- (c) the linking group is covalently bound to the organic polymer surface, the covalent bond resulting from the co-polymerization of an unsaturated moiety of a linker group precursor with a prepolymer of the organic polymer to form the linking group pendant to the surface;
- or
- (d) the organic polymer surface comprises a polyhydroxylated organic polymer comprising (co)polymerized polyvinyl alcohol, polyalkylhydroxy(meth)acrylate, polyhydroxyalkyl(meth)acrylamide, monoglycerol(meth)acrylate, monoglycerol(meth)acrylamide, or a combination thereof, or (co)polymerized (meth)acrylate linkages;
- or
- (e) the linking group is covalently bound to the hydroxylated inorganic surface.
Dependencies are inferred from the claim text.