Synergistic enhancement of the delivery of nucleic acids via blended formulations
AssigneeTranslate Bio, Inc.
InventorsFrank DeRosa, Lianne Smith, Michael Heartlein, Braydon Charles Guild
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Abstract
Disclosed herein are pharmaceutical compositions that comprise “blends” of lipid nanoparticles and related methods of using such blended compositions to deliver polynucleotides to one or more target cells, tissues or organs. The blended compositions are generally characterized as being able to efficiently deliver polynucleotides to target cells and by their ability to enhance the expression of such polynucleotides and the production of functional proteins by target cells.
Claims14
A method of delivering a messenger RNA (mRNA) to a subject, comprising administering to the subject a single pharmaceutical composition comprising a blend of at least a first and a second separately formed, non-identical lipid nanoparticles, each lipid nanoparticle comprising one or more PEG-modified lipids,
- wherein the first lipid nanoparticle comprises a first mRNA and the second lipid nanoparticle comprises a second mRNA,
- wherein the first lipid nanoparticle comprises a first cationic lipid and the second lipid nanoparticle comprises a second cationic lipid,
- wherein the first cationic lipid and the second cationic lipid are non-identical, and
- wherein an expression of a protein or a peptide encoded by the first mRNA or the second mRNA following the administration of the pharmaceutical composition to the subject exceeds the expression of the protein or the peptide encoded by the first mRNA or the second mRNA administered with the first lipid nanoparticle or the second lipid nanoparticle respectively, but without the other lipid nanoparticle by at least about two-fold.
Dependencies are inferred from the claim text.