# US 10,640,798 B2 · Microorganisms for producing diamine and process for producing diamine using them

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- Number: US 10,640,798 B2
- Document ID: US10640798B2
- Status: Granted
- Kind: B2
- Application number: 16/000,619
- Filed: 2018-06-05
- Published: 2018-09-27
- Granted: 2020-05-05
- Earliest claimed: 2014-04-25
- Assignees: Cj Cheiljedang Corporation
- Applicants: Cj Cheiljedang Corporation
- Inventors: Kyoung Min Lee; Su Jin Park; Hee Kyoung Jung; Young Lyeol Yang; Hong Xian Li; Hye Won Um
- Web page: https://patentgrep.com/patent/US10640798B2

## CPC

- C12P 13/001: {Amines; Imines}
- C12N 1/20: Bacteria; Culture media therefor
- C12P 13/00: Preparation of nitrogen-containing organic compounds
- C12Y 203/01057: Diamine N-acetyltransferase (2.3.1.57)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

The present invention relates to a microorganism for producing diamine, in which activity of a protein having an amino acid sequence of SEQ ID NO: 6 or an amino acid sequence having 42% or higher sequence homology with SEQ ID NO: 6 is introduced or enhanced, and a method of producing diamine using the same.

## Claims

### Claim 1

A microorganism for producing cadaverine, wherein an activity of a protein comprising an amino acid sequence having at least 95% identity to SEQ ID NO:23, or SEQ ID NO:26 is introduced or enhanced compared to the endogenous activity.

### Claim 2 (depends on 1)

The microorganism according to claim 1, wherein an activity of diamine acetyltransferase is further weakened compared to the endogenous activity.

### Claim 3 (depends on 2)

The microorganism according to claim 2, wherein the diamine acetyltransferase has the amino acid sequence selected from the group consisting of SEQ ID NOS: 12, 13, and 14.

### Claim 4 (depends on 1)

The microorganism according to claim 1, wherein the microorganism is a microorganism belonging to genus Corynebacterium or genus Escherichia.

### Claim 5 (depends on 4)

The microorganism according to claim 4, wherein the microorganism is a microorganism belonging to genus Corynebacterium to which an activity of lysine decarboxylase is further introduced.

### Claim 6 (depends on 1)

A method of producing cadaverine, comprising:
(i) culturing the microorganism of claim 1 to obtain a cell culture; and
(ii) recovering cadaverine from the cultured microorganism or the cell culture.

### Claim 7 (depends on 6)

The method according to claim 6, wherein in the microorganism, an activity of diamine acetyltransferase is further weakened compared to the endogenous activity.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the diamine acetyltransferase has the amino acid sequence selected from the group consisting of SEQ ID NOS: 12, 13, and 14.

### Claim 9 (depends on 6)

The method according to claim 6, wherein the microorganism is a microorganism belonging to genus Corynebacterium or genus Escherichia.

### Claim 10 (depends on 9)

The method according to claim 9, wherein the microorganism is a microorganism belonging to genus Corynebacterium to which an activity of lysine decarboxylase is further introduced.