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US 10,640,756 B2Granted
Method of producing monomeric card in bacteria
AssigneeKorea Institute of Science and Technology
InventorsHak Suk Chung, Jinsu An, Eun Gyeong Yang, So Yeon Kim
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Abstract
Provided is a method of producing monomeric caspase activation and recruitment domains (CARDs) in bacteria. According to this method, CARD domains may be easily produced in a monomeric form. Monomeric CARDs may be applied to studies on the mechanism of action of caspase, the development of new drugs, detection and elimination of endotoxins, development of cosmetics, etc.
Claims16
A method of producing monomeric caspase activation and recruitment domains (CARDs) in bacteria, the method comprising:
- introducing a vector comprising a polynucleotide encoding a caspase (Casp) protein into bacteria to prepare transformed bacteria;
- culturing the transformed bacteria to induce expression of caspase;
- lysing the cultured bacteria to obtain a lysate;
- and
- obtaining monomeric CARDs from the lysate.
2 more elements
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