Method for preparing composition for promoting hair growth using Nanog-introduced mesenchymal stem cells derived from fetus in amniotic fluid

A method for preparing a composition for promoting hair growth, which comprises a conditioned medium containing a basic fibroblast growth factor (bFGF), an insulin-like growth factor (IGF), wingless-type MMTV integration site family member 7A (Wnt7a) and a platelet-derived growth factor (PDGF-AA) from Nanog-overexpressing mesenchymal stem cells derived from a fetus in amniotic fluid as an active ingredient, the method comprising:

• (a) isolating fetal cells from the amniotic fluid obtained from a pregnant woman;
• (b) obtaining mesenchymal stem cells derived from a fetus in amniotic fluid by subculturing the isolated fetal cells in a culture medium containing fetal bovine serum (FBS), bFGF, selenium and ascorbic acid;
• (c) obtaining Nanog-overexpressing mesenchymal stem cells derived from a fetus in the amniotic fluid by introducing Nanog into the obtained mesenchymal stem cells derived from a fetus in amniotic fluid;
• (d) preparing a conditioned medium containing bFGF, IGF, Wnt7a and PDGF-AA by culturing the obtained Nanog-overexpressing mesenchymal stem cells derived from a fetus in amniotic fluid in a serum-free medium for 1 to 5 days;
• and
• (e) preparing a composition comprising the prepared conditioned medium as an active ingredient.