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US 10,639,597 B2Granted
Microfluidic devices
AssigneeBio-Rad Laboratories, Inc.
InventorsDarren Roy Link, Michael Weiner, David Marran, Jonathan M. Rothberg
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Abstract
The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
Claims16
A method of analyzing a compound released from a cell, the method comprising:
- providing a microfluidic substrate comprising an inlet module comprising a first inlet channel comprising a plurality of cells in aqueous solution and a second inlet channel comprising a plurality of primers in aqueous solution;
- flowing the aqueous solutions together to form a combined aqueous solution, and flowing the combined aqueous solution through a nozzle into a reservoir comprising an oil, thereby extruding a plurality of droplets of aqueous solution into the reservoir, each droplet comprising primers and at least one cell from the plurality of cells;
- incubating the plurality of the droplets off of the microfluidic substrate;
- causing at least one secreted substance to be released from the cell in each droplet;
- and
- conducting a homogenous assay with the primers on the secreted substance, the homogenous assay being conducted inside each of the plurality of droplets.
3 more elements
Dependencies are inferred from the claim text.